Figure 10.

Kinetics analysis for single transition DNA threading intercalation, from (43). (A) The on rates (k_on, green data points) and off rates (k_off, red data points) at Δ,Δ-P concentration of 5 nM fitted to a single exponential dependence on force (dotted lines) to obtain the distances related to the on and off transitions. (B) The on rates (k_on) and off rates (k_off) as a function of concentration at 30 pN force, and the dotted lines are linear fits. (C) The association rates k_a(F) (data points) for forces from 20 to 60 pN fitted to the exponential force-dependence (dashed line), yielding the zero-force association rate and the length change required for ligand association.