Table 2. DNA intercalation equilibrium parameters for unconventional intercalators investigated by single-molecule force spectroscopy.
| Equilibrium parameter | (Δ,Δ-Pc)4+ | (Δ,Δ-P)4+ | Thiocoraline | ActD | Triostin A |
|---|---|---|---|---|---|
| Kd(0) (nM) | 15 ± 6 | 44 ± 2 | 77 ± 14a | 1200 ± 500 | 1700 ± 0.002 |
| Δxeq (nm) | 0.44 ± 0.04 | 0.19 ± 0.01 | 0.41 ± 0.01 | 0.20 ± 0.01 | 0.32 ± 0.01 |
| Persistence length, P (nm) | 3 ± 1 | 2 ± 0.2 | 8 ± 1 | 10 ± 3 | |
| Elastic modulus, S (pN) | 800 ± 50 | 598 ± 30 | 745 ± 320 | 320 ± 20 | |
| Intercalative site size n (bp) | 3.2 ± 0.3b | 3.7 ± 0.1, (10 pN) | 3.8 ± 0.1, (2 pN) | 3.8 ± 0.1 | 3.8 ± 0.1, (10 pN) |
Non-zero force values are specified, where value (a) is Kd(F = 2 pN) and value (b) is determined by Equation (8) from reported quantities.