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. 2016 Apr 20;44(9):4233–4242. doi: 10.1093/nar/gkw286

Figure 3.

Figure 3.

Agarose gel showing PCR products from leader ends of Sulfolobus islandicus CRISPR loci before and after conjugation. (A) Locus 1: (1) no pKEF9, (2) 22 hpc. (3) 37 hpc. Locus 2: (4) no pKEF9, (5) 22 hpc. (6) 37 hpc. (B) Locus 1: (1) no pKEF9, (2) 85 hpc. Locus 2: (3) no pKEF9 (4) 85 hpc. Arrows denote bands carrying de novo spacers. M—DNA size markers.