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. 2016 Apr 20;44(9):4233–4242. doi: 10.1093/nar/gkw286

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Analysis of pKEF9 integrated in the Sulfolobus islandicus genome. (A) Southern blot analysis of pKEF9 integration in S. islandicus. Lane 1—24 hpc; lane 2—38 hpc and lane 3 85 hpc. Bands 1, 2 and 3 derive from free pKEF9 and pKEF9 integrated at the tRNA^Glu (CTT) and tRNA^Glu (CTC) match sites, respectively. (B) PCR amplification products obtained at 85 hpc from pKEF9 integrated at lane 1—tRNA^Glu (CTC) and lane 2—tRNA^Glu (CTT). M—DNA size markers.