Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Agaose gels showing PCR products amplified from the five transposition sites of orfB elements in pKEF9: S1—14097/8; S2—17040/1; S3—22423/4; S4—26493/4 and S5—28710/1. (A) Donor strain. For each site: 1—free pKEF9 with full CRISPR locus; 2—pKEF9 from donor strain P1; 3—donor strain P1 DNA. (B) Recipient strain. For each site: 1—pKEF9 from donor strain P1; 2—donor strain P1 DNA and 3 recipient Sulfolobus islandicus DNA.
