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. 2016 May 19;6:26226. doi: 10.1038/srep26226

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(a) Phase contrast microscopy images of NMuMG cells and immunofluorescence staining of EMT markers at seeding densities of 5,000 cells/cm² and 100,000 cells/cm² with and without TGFβ1 treatment. Blue stain shows cell nuclei. Scale bars: 50 μm. (b) Western blot analysis of EMT markers for cells seeded at low (5,000 cells/cm²) and high (100,000 cells/cm²) densities with and without TGFβ1. (c) Mean cell area as a function of cell seeding density. (d) Percentage of cells expressing αSMA as a function of cell seeding density.