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. 2016 May 19;6:25806. doi: 10.1038/srep25806

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Copyright © 2016, Macmillan Publishers Limited

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Inhibition of hRSV RdRp by 8.3 μM of CPM, GDC-0449, tomatidine (a) and dilutions of CPM (b), measured by RdRp-specific minigenome assay in BSR-T7/5 cells. The cytotoxicity of CPM towards BSR-T7/5 cells is shown in (b). (c) Inhibition of hRSV replication complex by CPM measured by minigenome assay in BSR-T7/5 cells transfected with plasmids coding for a WT or a R151K variant M2-1. Viral RNA synthesis in (a–c) was quantified by measuring the luciferase activity after cell lysis 24 h after transfection. The error bars represent the standard error of the mean of luciferase activity measured in triplicates, normalized against β-galactosidase activity and expressed as percentage of a control treated with PBS. For the data points at 0 μM, DMSO was used in place of CPM. The results are representative of 2 independent experiments performed in triplicate.