Immunohistochemical staining for fibrillin-1 in uncultured normal pulp (A) and after 7 days of culture (B). In uncultured normal pulp, fibrillin-1 is densely detected in the dental pulp (A) and within the interodontoblastic area (A, inset). In contrast, after 7 days of culture, fibrillin-1 staining becomes rather faint (B), except around blood vessels (B, arrows) and the interodontoblastic area is devoid of fibrillin-1 (B, insert). A negative control section shows no specific immunoreactivity (C). OB, odontoblast. Scale, 50 µm.