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. 2015 Mar 24;63(6):438–448. doi: 10.1369/0022155415580622

Figure 4.

Figure 4.

Quantitative RT-PCR analysis of fibrillin-1 and α-SMA. Fibrillin-1 mRNA levels decrease by almost half at 7 days of culture as compared with that in uncultured tissues. In contrast, α-SMA mRNA levels are significantly increased at 7 days of culture as compared with that in uncultured tissues, and this increase is significantly attenuated by protease inhibitors (4-Abz-Gly-Pro-D-Leu-D-Ala-NH-OH and P1860) and by the inhibitor of TGF-β type I receptor (SB431542). mRNA expression levels were normalized to GAPDH mRNA levels. Significant difference against uncultured normal (*) and day 7 (**) without inhibitors in fibrillin-1 mRNA expression. *p<0.05.