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Immunohistochemical staining and quantitative RT-PCR analysis of TGF-β1. TGF-β1 is broadly observed in uncultured normal pulp (A). TGF-β1 mRNA levels are significantly decreased at 2 and 3 days (B). Protease inhibitors (4-Abz-Gly-Pro-D-Leu-D-Ala-NH-OH and P1860) do not affect TGF-β1 mRNA levels significantly (B). TGF-β1 mRNA expression levels normalized to GAPDH mRNA levels. OB, odontoblast. *p<0.05 compared with uncultured normal pulp. Scale, 50 µm.
