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. 2015 Mar 24;63(6):438–448. doi: 10.1369/0022155415580622

Figure 6.

Figure 6.

Immunohistochemical staining of pSmad2/3 in cultured pulp tissue without protease inhibitors and the mean number of pSmad2/3-positive nuclei. (A) Time-dependent translocation of pSmad2/3 is observed in the cytoplasm at 1 day and into the nucleus at 2, 3, and 4 days. At 5 days, pSmad2/3 is diffusely noted in the cytoplasm but no immunoreactivity for pSmad2/3 is observed at 7 days. The number of pSmad2/3-positive nuclei peaks at 3 days of culture. (B) The mean number of pSmad2/3-positive nuclei per field (×400) ± SE. n=4. *p<0.05 and **p<0.01 compared with uncultured normal pulp. Scale, 10 µm.