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. 2016 May 19;6:26418. doi: 10.1038/srep26418

Figure 3. Determination of cortisone and cortisol by HPLC-MS/MS.

Figure 3

(A) Biological activity of 11β-HSD1. (B) HPLC-MS/MS Chromatogram of cortisone and cortisol, (C,D) Positive ESI MS/MS spectra acquired on an API4000 mass spectrometer for cortisol with collision energy of 33 eV and cortisone with collision energy of 30 eV.