Table 3. Identification of the five well-known proteins associated with cellulose synthase complex in cotton fibers by LC-MS/MS analysis.
| Protein name |
Total peptides (Exp 1) |
Total peptides (Exp 2) |
Total peptides (Exp 3) |
Accession number | TM domain | Protein fuction | |||
|---|---|---|---|---|---|---|---|---|---|
| Blank (B) | Treated (T) | Blank (B) | Treated (T) | Blank (B) | Treated (T) | ||||
| alpha-tubulin 4 | 20(10)@ | 22(10) | 14(6) | 14(7) | 0(0) | 12(9) | gi|37529490 | 0 | Cytoskeleton, GTPase activity, guidance of cellulose deposition25,26,30 |
| beta-tubulin 3 | 25(10) | 23(10) | 30(10) | 19(8) | 33(11) | 0(0) | gi|223453022 | 0 | Cytoskeleton, GTPase activity, protein binding, guidance of cellulose deposition25,26,30 |
| beta-tubulin 7 | 19(7) | 23(8) | 33(12) | 20(8) | 37(11) | 32(12) | gi|37529498 | 0 | Cytoskeleton, GTPase activity, protein binding, guidance of cellulose deposition25,26,30 |
| endo-1,4-beta-glucanase (KOR) | 3(2) | 2(2) | 21(8) | 14(5) | 23(12) | 25(13) | gi|32454474 | 1 | cellulase activity, cellulose biosynthesis21,22,24,32,33,38,57 |
| sucrose synthase 1 | 6(3) | 4(2) | 10(6) | 9(5) | 20(13) | 16(12) | gi|258489633 | 0 | UDP-glycosyltransferase activity, cellulose biosynthesis20,23,31 |
(B)/(T): Treated without/with 1% cellulose.
@Indicated total peptide counts and distinct peptide numbers (brackets); TM: Transmembrane.