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. 2016 May 19;6:26424. doi: 10.1038/srep26424

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Copyright © 2016, Macmillan Publishers Limited

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(a) Monocyte gating strategy. Typical light-scatter density-plot of PBMCs (left) and CD14^highCD16^low monocytes were isolated from the monocyte-containing gate based on CD14 and CD16 expression (right). (b) Isolated CD14^highCD16^low monocyte population (96.7% ± 1.0% purity). (c) Cell numbers (left) and DNA (middle) and RNA (right) yields obtained for each cell population. (d) Estimated cellular composition for each sample as determined by reference information on cell-specific DNA methylation signatures using the “estimateCellCounts” function implemented in the minfi package. Bars in (c,d) represent mean ± standard deviation (grey, participant #1; black, participant #2).