Fig. 4. Migration of alloreactive effectors in adoptive hosts of CD8 T_CM and T_EM.

Sorted CD8 T_CM (2 × 10⁶) or T_EM (0.5 × 10⁶) containing similar numbers of BALB/c-reactive IFNγ⁺ T cells were transferred to wt and aly/aly-spleen mice followed by BALB/c-skin transplantation 2-days later. Alloreactive effectors from CD8 T_CM and T_EM were assessed after BALB/c-skin transplantation. Cells were harvested from liver (LV), lungs (LG), bone marrow (BM), blood and skin allograft (SK) in both wt and aly/aly-spleen hosts. Spleen (SP), draining lymph node (dLN) and non-draining LN cells were also harvested in wt hosts. BALB/c-reactive IFNγ⁺ T cells within the harvested cells from all tissues were assessed by intracellular cytokine staining after gating on CD8⁺Thy1.1⁺ population to identify alloreactive effectors derived from CD8 T_CM and T_EM. (A – B) Tissue distribution of alloreactive effector T cells generated from transferred CD8 T_CM and T_EM in wt (A) and aly/aly-spleen (B) hosts. CD8⁺Thy1.1⁺ IFNγ⁺ T cells harvested from each tissue is shown as % of total CD8⁺Thy1.1⁺ IFNγ⁺ T cells harvested from all tissues in that recipient (Mean ± SD of 3 – 4 mice/grp). CD8⁺ Thy1.1⁺ IFNγ⁺ T cells harvested from blood and non-draining LN were < 1% of total and are not shown. (C – D) Quantitation of BALB/c-reactive IFNγ⁺ T cells within the harvested CD8⁺ Thy1.1⁺ population from skin allografts in either wt (C) or aly/aly-spleen (D) adoptive hosts of CD8 T_CM and T_EM (Mean ± SD of 3 – 4 mice/grp). (E) Tissue distribution of alloreactive effector T cells generated from transferred CD8 T_CM and T_EM in aly/aly-spleen hosts at 30-days after BALB/c-skin transplantation (similar to 4B). (F) Quantitation of BALB/c-reactive IFNγ⁺ T cells within the harvested CD8⁺ Thy1.1⁺ population from skin allografts in aly/aly-spleen adoptive hosts of CD8 T_CM and T_EM at 30-days after BALB/c-skin transplantation.