Fig. 3.

Buffy rescues the α-synuclein-induced phenotypes of decreased lifespan and climbing ability. a Directed overexpression of Buffy in the DA neurons increase longevity whereas flies with Buffy loss-of-function show a decline in lifespan. Genotypes are UAS-α-synuclein, Ddc-Gal4/UAS-lacZ; UAS-α-synuclein, Ddc-Gal4/UAS-Buffy; and UAS-α-synuclein, Ddc-Gal4/UAS-Buffy-RNAi. Longevity is shown as percent survival (P < 0.01, determined by log-rank and n ≥ 200). b The co-expression of Buffy in the α-synuclein model of PD rescued the age-dependent loss in climbing ability. The directed overexpression of Buffy in the DA neurons remarkably increased the climbing ability over time compared to the control, while the suppression of Buffy resulted in flies that climbed similar to the control. The genotypes are UAS-α-synuclein; Ddc-Gal4/UAS-lacZ, UAS-α-synuclein; Ddc-Gal4/UAS-Buffy, and UAS-α-synuclein; Ddc-Gal4/UAS-Buffy-RNAi. Analysis of the climbing curves and significance was determined by comparing the 95 % confidence intervals. Error bars indicate the SEM, asterisks represents statistically significant result and n = 50