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. 2016 May 18;13:111. doi: 10.1186/s12974-016-0577-8

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© Marignier et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Chronic infusion of IgG^AQP4+ decreased AQP4 expression in the rat spinal cord and optic nerve. a Example of AQP4 loss with preserved GFAP-positive astrocytes in the spinal cord of the NMO-rat compared to the Control-rat (D7, rat infused with IgG^AQP4+1,2 and IgG^Control1,2 as an example) IHC immunohistochemistry. Scale bars = 20 μm. b Confirmation of AQP4 loss using evaluation of AQP4 expression in the white and gray matter of the NMO-rat spinal cord (n = 5) compared to the Control-rat (n = 4), by IHC (fluorescence intensity) and Western blot (Wb, relative to actin). In parallel, GFAP expression is enhanced (Wb). c Loss of AQP4 expression in the optic nerve of the NMO-rat as shown by IHC and evaluation of fluorescence intensity (*p < 0.05; ***p < 0.001, ANOVA)