Figure 1.

Chemiluminescence assay represents the production of reactive oxygen species (ROS) in leucopathes during 60 min of stimulation with Pseudomonas aeruginosa PAO1, zymosan‐activated serum (ZAS) or the protein kinase C agonist phorbol myristate acetate (PMA). Diphenylene iodonium (DPI) a specific inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, was employed as control in one experiment. The luminol‐enhanced NADPH oxidase is employed as control. Results are expressed as counts per second (cps) max ± standard deviation (s.d.). Experiments were performed in triplicate. *P < 0·0005.