Figure 5.

Chemotaxis assay describe the chemotactic migration of leucocytes residing in the leucopatch. Formyl‐methionyl‐leucyl‐phenylalanine (fMLP) and Pseudomonas aeruginosa (PAO1) were applied as leucocyte chemoattractants/activators. The identification and the quantity of leucocytes migrated through 5‐μm pore size polycarbonate filters were determined by flow cytometry using fluorescein isothiocyanate (FITC)‐conjugated mouse anti‐human CD15, allophycocyanin (APC)‐conjugated mouse anti‐human CD14 and peridinin chlorophyll (PerCP)‐conjugated mouse anti‐human CD45 monoclonal antibodies. Experiments were performed twice.