Figure 3.

Effects of minocycline (MINO) on the locomotor responses to acute challenge with psychotomimetic drugs in adult offspring exposed to prenatal control treatment or immune activation with additional stress exposure in puberty. Pregnant mice were injected with 1 mg kg⁻¹ poly(I:C) (POL) or physiological saline (control (CON)), and the resulting offspring were subjected to sub-chronic stress (S+) during peripubertal development. During the stress procedure, half of the animals received MINO treatment (30 mg kg⁻¹ per day, per os in drinking water), and the other half vehicle (VEH; = regular tap water) treatment. (a) Locomotor reaction to the indirect dopamine receptor agonist amphetamine (Amph; 2.5 mg kg⁻¹, i.p.). The line plot depicts the distance moved in an open field arena to initial vehicle (saline (Sal)) treatment and subsequent Amph treatment as a function of successive 5-min bins. The bar plot depicts the mean distance moved after Amph treatment. **P<0.01 and ***P<0.001; N=12–16 per group. (b) Locomotor reaction to the non-competitive NMDA receptor antagonist dizocilpine (MK-801; 0.15 mg kg⁻¹, i.p.). The line plot shows the distance moved in an open field arena to initial vehicle (Sal) treatment and subsequent MK-801 treatment as a function of successive 5-min bins. *P<0.05, reflecting the significant difference between VEH-exposed CON/S+ and POL/S+ offspring at individual bins; ^§P<0.05, reflecting the significant difference between VEH-exposed POL/S+ and MINO-treated POL/S+ offspring at individual bins. N=16 per group. All data are means±s.e.m.