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. 2016 Feb 9;6(2):e733. doi: 10.1038/tp.2016.1

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Effect of restraint on the MPTP-dependent striatal cell loss and astrocytes activation. (a) Representative western blot and relative densitometric analyses of TH and GFAP immunoreactivity in striatum homogenates of the four groups of C57BL/6 mice receiving vehicle (CTR, n=10); MPTP (25 mg kg⁻¹ in saline intraperitoneally for a week; n=12); MPTP+RI (n=9); and RI (n=9; *P<0.05). Results are mean±s.e.m. (b) Representative images of immunohistochemistry analyses for TH and GFAP reactivity in the striatum region of the same groups of mice (scale bars, 20 μm (× 4); 100 μm (× 40)). GFAP, glial fibrillary acidic protein; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; RI, restraint/isolation; TH, tyrosine hydroxylase.