Table 2. Animal models of diet-induced obesity.

Study	High-fat diet	Metabolic factors	Study duration
Pagliassotti et al.122	60% of kcal from fat	Plasma level of insulin, β-hydroxybutrate, mRNA level and activity of lipoprotein lipase were higher in epididymal fat pad and lower in gastrocnemius muscle	5 Weeks
Huang et al.123	59% of kcal from fat	Increased mRNA levels of leptin receptor and neuropeptide Y. Decreased POMC mRNA level	13 Weeks
Dourmashikin et al.124	50% fat content	Increased levels of leptin, insulin and lipoprotein lipase in adipose tissue. Hyperphagia, enhanced circulating triglycerides, nonesterified fatty acids and enhanced glucose, galanin. Increased β-hyroxyacyl-coaldehydrogenase level in muscle	3 Weeks
Dourmashikin et al.125	45–60% fat content	Elevated leptin, insulin, triglyceride, glucose, lipoprotein lipase activity in adipose tissue	4–6 Weeks
Madsen et al.121	31.8% fat content	Development of visceral obesity, hyperleptinaemia, hyperinsulinemia and dyslipidaemia, and decrease in glucose tolerance	9 Months

Abbreviation: POMC, pro-opiomelanocortin.