Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant phenotype(s) or genotype(s) | Source or reference(s) |
|---|---|---|
| Strains | ||
| E. coli TG1 | supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK- mK-) F′ [traD36 proAB + lacIq lacZ_M15] | Sambrook and Russel, 2001 |
| E. coli Rosetta pLysS (DE3) | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CmR) | Novagen |
| L. salivarius IBB3154 | Isolated from chickens | Kobierecka et al., 2015 |
| C. jejuni 12/2 | Wild type; isolated from a chicken; good colonizer; pUOA18 (CmR) | Wyszyńska et al., 2004 |
| Plasmids | ||
| pGEM-T Easy | ApR; T vector for cloning PCR products | Promega |
| pET28a | KmR; lacI; overexpression vector | Novagen |
| pET22 | ApR; lacI; overexpression vector | Novagen |
| pBluescript II SK | ApR; general cloning vector | Stratagene |
| pUWM1287 | Source of fragment DNA encoding motif lysM L. lactis IL1413 | This work |
| pUWM1293 | 6xhis-lysM fusion in pET28b | This work |
| pUWM1312 | 6xhis-cjaA-lysM fusion in pET28a | Kobierecka et al., 2015 |
| pUWM1282 | 6xhis-cjaD-lysM fusion in pET28a | Kobierecka et al., 2015 |
| pUWM1379 | 6xhis-rcjaAD-6xhis fusion in pET28a | Kobierecka et al., 2016 |
| pUWM1414 | 6xhis-rcjaAD-lysM fusion in pET28a | This work |