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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Clin Cancer Res. 2009 Jan 15;15(2):492–501. doi: 10.1158/1078-0432.CCR-08-1945

Fig. 4.

Fig. 4

Input of c-Myc in transcriptional activation of VEGF. A, mutation of the c-Myc-binding site decreased VEGF promoter activity stimulated by LPA. Caov-3 cells were transfected with the wild-type reporter vector (pGL2-VEGF123-Luc) or c-Myc mutant vector (pGL2-VEGF123Mycmu-Luc). The transfected cells were starved and treated with LPA (10 μmol/L) or vehicle (BSA control) for 6 h and analyzed for luciferase activity. B, down-regulation of c-Myc expression attenuated VEGF promoter activity induced by LPA. Caov-3 cells were transfected with pGL2-VEGF123-Luc along with c-Myc-specific siRNA or nontarget control siRNA. LPA-stimulated luciferase activity was measured as in A. C, blockade of c-Myc with a specific inhibitor reduced the VEGF promoter activity. Caov-3 cells transfected with pGL2-VEGF123-Luc were stimulated in the absence or presence of 10058-F4 (2.5 μmol/L) and analyzed for luciferase activity.