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Trapping and conventional 3D imaging of single mouse embryonic stem cell (mESC) nuclei. White light and iRFP-tagged histone H2B images of the trapped nuclei are shown left and right respectively. The centromeres (imaged using mEos3-tagged Cenpa) are shown in the middle with each centromere colour-coded to represent its position in the z-axis after running the 3D stacks through custom-written deconvolution software.
