Fig. 2.

Localisation of RI_epac and RII_epac reporters. (A) Schematic representation of the FRET reporters RI_epac and RII_epac. The dimerisation/docking domains from PKA-RIα and PKA-RIIβ, which mediates anchoring of the sensors to specific AKAPs, are shown in white and black, respectively. (B) Left panels: confocal images illustrating the localisation of RI_epac or RII_epac (in green) and the reference marker sarcomeric α-actinin (in red) in ARVM expressing the sensor in culture for 24 h without any further treatment. Right panels: line intensity profiles for RI_epac and RII_epac (in green) and α-sarcomeric actinin (in red). The position of the line where the intensity values were calculated is shown in the corresponding magnified panels on the left. (C) Samples are as in A except that cells were treated for 24 h with NE (1 μM). (D) Pearson's correlation coefficient calculated for RI_epac or RII_epac and sarcomeric α-actinin in untreated and NE-treated ARVM. n ≥ 7. Data expressed as mean ± SEM. Two-way ANOVA with Bonferroni multiple comparisons tests was performed. Scale bars are 10 μm.