Skip to main content
. 2016 May 19;6:26244. doi: 10.1038/srep26244

Figure 3. Expression of CDK inhibitor and cyclin mRNAs in E8.0 Nczf−/− mice and immunohistochemical analysis at E6.5.

Figure 3

(A) Nczf, p53, p19, p21, p27, p57, p16, cyclin D1, cyclin D2, cyclin D3, cyclin E1, and cyclin E2 mRNA expression in wild type (closed bars) and Nczf−/− mice (open bars) at E8.0. Target-specific mRNA levels were assessed by real time PCR and normalized to Gapdh mRNA (n = 4–5). The expression in wild type mice was set to 1 and the relative amount of mRNA expressed in Nczf−/− mice was calculated. *P < 0.01. (B) Whole images of E6.5 embryos from wild type and Nczf−/− mice immunostained with p27, PCNA, BrdU and active caspase3 antibodies. Scale bar: 100 μm. Abbreviations; Pe: Primitive ectoderm, Ee: Extra embryonic ectoderm, Md: Mesoderm.