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. 2016 May 19;6:26277. doi: 10.1038/srep26277

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Cells were treated with 1 mM bupivacaine (Bupi) for 24 h followed by immunostaining and western blot. (A) Caspase 3, (B) Caspase 8, (C) Caspase 9 expression in SKOV-3 cells. (D) Caspase 3, (E) Caspase 8, (F) Caspase 9 expression in PC-3 cells. (G) Cleaved caspase 3 expression in SKOV-3 and PC-3 by western blot. Data are presented as mean ± SD. (n = 4). ***P < 0.001. Scale bar = 50 μm.