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. 2016 May 19;6:26277. doi: 10.1038/srep26277

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SKOV-3 cells transfected with siRNA were treated with 1 mM bupivacaine for 24 h. (A) pGSK-3β^tyr216 (red) and caspase 8, 9 (green) expression in SKOV-3. (B) pGSK-3β^tyr216 (red) and caspase 3 (green) co-expression. (C) The fluorescent intensity analysis of pGSK-3β^tyr216 and cleaved caspase 3, 8 and 9. Data are presented as mean ± SD (n = 5). *P < 0.01; **P < 0.001. Scale bar = 50 μm.