A) Cyclophilin D (CypD) transgenic mice (+) and nontransgenic (nonTg, −) control mice were identified by PCR results. B-C) Immunoblotting of brain homogenates from Tg CypD (lane 2, +) mice and nonTg littermate controls (lane 1, −) for CypD, using anti-human CypD antibody. C) Quantification of CypD immunoreactive bands normalized to β-actin. Data are presented as fold increase relative to nonTg mice. N = 5-6 mice/group. D-F). The double immunofluorescent staining of brain sections for CypD (red) and MAP2 (green) in hippocampus (D) and cortex (E) from the indicated Tg mice. Nuclei were stained by DRAQ5 as shown in blue. F) Quantification of CypD staining intensity in hippocampus and cortex regions of the indicated Tg mice. G) Representative immunostaining images for CypD (green) and SODII (red, mitochondrial marker) and nuclei (blue) in hippocampal and cortical neurons. Scale bar = 25 μm.