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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jul 29;1852(10 Pt A):2225–2234. doi: 10.1016/j.bbadis.2015.07.019

Figure 5. The effect of p53 and NR2B activation on OGD-induced CypD/p53 complex formation in nonTg mice.

Figure 5

A) Immunoprecipitation of hippocampal homogenates with p53 antibody followed by immunoblotting with CypD antibody revealed CypD immunoreactive bands at 53kD in OGD-exposed non-transgenic (nonTg) hippocampal tissue. CypD/p53 complex was elevated in CypD-overexpressed mice and absent in CypD-deficient animals. β-actin bands show the equal amounts of protein used for Co-immunoprecipitation experiments. B). Pifithrin-μ (PFT, 5μM) treatment significantly suppressed OGD-induced CypD/p53 interaction in mitochondrial fractions. C) Inactivation of NR2B subunit instead of NR2A prevented CypD/p53 interaction from the isolated brain mitochondria after OGD. Experiments repeated at least 3 times; 5-6 mice per group. PFT: pifithrin-μ; Ro25: Ro25-6981