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. 2016 May 19;6:26227. doi: 10.1038/srep26227

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Copyright © 2016, Macmillan Publishers Limited

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Corneal stroma graft (*) isolated from the central part of the cornea was cultured in a cell culture plate (A), giving rise to elongated cells in the first 14 days of in vitro culture. Cells formed a monolayer by day 28, and showed fibroblastoid morphology according to their cytoskeletal structure. The expression of carbohydrate molecules on the surface of CSMSCs labelled by fluorescein-conjugated lectins is being shown (B). GSL I:Griffonia (Bandeiraea) simplicifolialectin I (Griffoniasimplicifolia); LCA:Lens culinaris agglutinin (Lens culinaris); PHA E:Phaseolus vulgaris erythroagglutinin (Phaseolus vulgaris); PHA L:Phaseolus vulgaris leucoagglutinin (Phaseolus vulgaris); PSA:Pisumsativum agglutinin (Pisumsativum; sWGA: succinylatedWheat germ agglutinin (Triticum vulgaris);DBA: Horse gram lectin/Dolichosbiflorus agglutinin (Dolichosbiflorus); ConA: Concanavalin A (Canavaliaensiformis);PNA: Peanut agglutinin (Arachishypogaea); RCA 120:Ricinus communis agglutinin (Ricinuscommunis);SBA: Soy bean agglutinin (Glycine max); UEA:Ulexeuropaeus agglutinin (Ulexeuropaeus); WGA: Wheat germ agglutinin (Triticum vulgaris); AIL:Jacalin(Artocarpusintegrifolia) (Magnification: 100X A1-3; 200XB1-14, Phalloidin-TRITC staining A-3, 400× Lectin staining B1-14).