Table 1. Expression of carbohydrate molecules on the surface of in vitro cultured CSMSCs.
| Lectin | Positive Cells (%) | Sugar Specificity |
|---|---|---|
| ConA | 80.38 ± 5.36 | α-mannose, α- D-glucose |
| GSL I | 0.00 ± 0.00 | α- D-galactose, α linked N-acetylgalactosamine |
| AIL | 90.86 ± 1.85 | (Sialic Acid)β1 D-galactose -3 α1 N-acetylgalactosamine |
| WGA | 92.19 ± 2.34 | α- or β-linked N-acetylglucosamine |
| UEA | 0.00 ± 0.00 | α1 L-fucose-2 D-galactose |
| SBA | 8.15 + 5.66 | β1 N-acetylglucosamine – 4 β1 N-acetylglucosamine, 4 N-acetylglucosamine - N-acetylneuraminic acid (sialic acid) |
| RCA | 84.35 ± 6.11 | α- or β-linked N-acetylgalactosamine |
| PNA | 6.31 ± 5.68 | β1 D-galactose −3 α1 N-acetylgalactosamine |
| DBA | 1.10 ± 0.72 | N-acetylgalactosamine |
| succinylated WGA | 80.93 ± 8.21 | N-acetylglucosamine |
| PSA | 81.94 ± 6.26 | α-mannose, α- D-glucose |
| PHA-L | 78.00 ± 7.23 | β4 D-galactose-4 β6 N-acetylglucosamine (β2N-acetylglucosamine - α3 mannose) α3 mannose |
| PHA-E | 86.50 ± 3.76 | β4 D-galactose - β2N-acetylglucosamine - α6 mannose (β4 N-acetylgalactosamine) β4 N-acetylgalactosamine - α3 mannose) – β4 mannose |
| LCA | 84.00 ± 5.51 | α-mannose, α- D-glucose |
Majority of the CSMSCs contained mannose, glucose, poly-saccharides with N-acetylglucosamine and N-acetylgalactosamine molecules, and lacked fucose, based upon the specific lectin screening. These carbohydrate molecules could determine the extracellular matrix and/or cell-cell binding and immunological properties of the cells (Data shown are mean ± SEM, N = 8; for abbreviations see Fig. 1).