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. 2016 Apr 15;88(10):5053–5057. doi: 10.1021/acs.analchem.6b00381

Figure 3.

Figure 3

Release of mRNA under various conditions for M. smegmatis. The released copy number per cell for 4 mRNAs was quantified using qRT-PCR (n = 3). The copy per cell values for microfluidics-produced samples were calibrated by deducting the fraction generated by DNA templates in the cell lysate (Figure S5). (a) The effect of pulse numbers and comparison to bead beating. Various numbers of pulses (5 s duration for each pulse with 6000 V/cm intensity) were applied. (b) The effect of pulse intensity. In each case, 30 pulses (5 s each) were applied.