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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Biochem J. 2008 May 1;411(3):543–551. doi: 10.1042/bj20071299

Figure 1. Selective expression of LPA3 receptors and stimulation of Gαq-mediated PI hydrolysis, Rho kinase and PKC by LPA in gastric smooth muscle.

Figure 1

(A) Lysates were prepared from freshly dispersed smooth muscle cells (fsm), cultured smooth muscle cells (csm) and brain (b). The proteins were resolved by SDS/PAGE and transferred on to PVDF membranes and were detected with specific antibodies to LPA1, LPA2 or LPA3 receptors. (B) Cultured muscle cells expressing the Gαq or Gαi minigene were treated with LPA for 60 s. In all experiments, the cells were prelabelled with myo-[3H]inositol. (C) Rho kinase activity was measured by immunokinase assay as described in the Experimental section. (D) PKC activity was measured in membrane fractions using myelin basic protein as the substrate. Results were expressed as c.p.m./mg of protein. Values are means ± S.E.M. of four experiments. **P < 0.01 significant stimulation of PI hydrolysis, Rho kinase or PKC by LPA.