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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Biochem J. 2008 May 1;411(3):543–551. doi: 10.1042/bj20071299

Figure 6. LPA-induced phosphorylation of MYPT1 at Ser695 and activation of the NF-κB pathway.

Figure 6

Dispersed muscle cells were incubated for 10 min with H-89 (1 μM), myristoylated PKI (1 μM), MG-132 (10 μM) or for 2 h with C3 exoenzyme (2 μg/ml) and then treated with LPA (1 μM) or forskolin (FSK, 10 μM) for 5 min. (A) Phosphorylation of MYPT1 was measured using a phospho-specific Ser695 antibody. ‘+’ indicates addition of inhibitors in the presence of LPA. (B) IKK2 phosphorylation was measured using a phospho-specific Ser177/181 antibody and IκBα degradation was measured using an IκBα antibody. (C) Cultured muscle cells expressing control vector or IKK2(K44A) were treated with LPA for 5 min. Phosphorylation of MYPT1 and IKK2, and degradation of IκBα were measured as described above.