Table 5.

Respiratory parameters of glutamate/malate-respiring rat heart mitochondria (0.5 mg protein per mL) in the absence (Control) or in the presence of 1 mM L-tryptophan (L-TRP) or its metabolites L-kynurenine (L-KYN), kynurenic acid (KYNA), anthranilic acid (ANA), 3-hydroxykynurenine (3-OH-KYN), 3-hydroxyanthranilic acid (3-OH-ANA), xanthurenic acid (XAN) and quinolinic acid (QUIN), respectively.

SUBSTANCES	STATE 2	STATE 3	RC	ADP/O
Control	22.4 ± 1.8	151 ± 11	6.81 ± 0.32	2.45 ± 0.09
L-TRP	22.5 ± 2.0	142 ± 11	6.34 ± 0.29	2.49 ± 0.11
QUIN	22.3 ± 2.2	143 ± 11	6.56 ± 0.38	2.47 ± 0.10
ANOVA, F	0.0034	0.219	0.506	0.046
P	0.997	0.806	0.613	0.955
Control	23.7 ± 1.7	150 ± 13	6.32 ± 0.29	2.63 ± 0.07
L-KYN	23.3 ± 1.5	145 ± 15	6.18 ± 0.31	2.63 ± 0.07
3-OH-KYN	23.2 ± 1.6	94.7 ± 8.6**	4.07 ± 0.21***	2.48 ± 0.07
ANOVA, F	0.022	6.117	21.191	1.393
P	0.978	0.011	<0.001	0.279
Control	20.9 ± 1.0	123.7 ± 5.5	5.97 ± 0.28	2.51 ± 0.04
KYNA	24.8 ± 0.9*	117.4 ± 4.1	4.75 ± 0.15**	2.43 ± 0.05
ANA	21.7 ± 0.8	88.6 ± 1.7***	4.09 ± 0.12***	2.51 ± 0.07
ANOVA, F	5.418	20.955	24.161	0.798
P	0.017	<0.001	<0.001	0.469
Control	21.0 ± 0.6	127.8 ± 7.8	6.09 ± 0.29	2.64 ± 0.05
3-OH-ANA	25.4 ± 1.0**	96.4 ± 3.8**	3.82 ± 0.12***	2.30 ± 0.08**
XAN	22.6 ± 0.6	126.8 ± 8.8	5.61 ± 0.31	2.56 ± 0.08
ANOVA, F	9.341	6.213	21.843	6.530
P	0.0023	0.011	<0.001	0.0091

Notes: States are given in nmol O × min⁻¹ × mg⁻¹. Values represent means ± S.E.M; n = 6;

^*P < 0.05,

^**P < 0.01

^***P < 0.001; in comparison to the respective control.