Skip to main content
. 2016 May 17;9:17–29. doi: 10.4137/IJTR.S37973

Table 6.

Respiratory parameters of rotenone-inhibited succinate-respiring rat heart mitochondria (0.25 mg protein per mL) in the absence (Control) or in the presence of 1 mM L-tryptophan (L-TRP) or its metabolites L-kynurenine (L-KYN), kynurenic acid (KYNA), anthranilic acid (ANA), 3-hydroxykynurenine (3-OH-KYN), 3-hydroxyanthranilic acid (3-OH-ANA), xanthurenic acid (XAN) and quinolinic acid (QUIN), respectively.

SUBSTANCES STATE 2 STATE 3 RC ADP/O
Control 81.3 ± 2.3 262 ± 10 3.25 ± 0.10 1.34 ± 0.04
L-TRP 84.5 ± 3.1 268 ± 11 3.18 ± 0.07 1.34 ± 0.04
QUIN 82.5 ± 2.9 254 ± 11 3.07 ± 0.05 1.30 ± 0.04
ANOVA, F 0.342 0.476 1.301 0.404
P 0.715 0.630 0.301 0.675
Control 77.8 ± 6.2 269 ± 23 3.46 ± 0.08 1.31 ± 0.03
L-KYN 80.9 ± 7.6 270 ± 26 3.33 ± 0.03 1.27 ± 0.03
3-OH-KYN 79.6 ± 7.3 242 ± 24 3.05 ± 0.05** 1.23 ± 0.04
ANOVA, F 0.048 0.400 13.050 1.673
P 0.9538 0.677 <0.001 0.221
Control 88.0 ± 3.0 265 ± 10 3.03 ± 0.04 1.18 ± 0.04
KYNA 95.2 ± 6.2 260 ± 11 2.78 ± 0.12 1.19 ± 0.02
ANA 90.1 ± 6.7 262 ± 13 2.96 ± 0.12 1.21 ± 0.04
ANOVA, F 0.446 0.046 1.702 0.235
P 0.648 0.955 0.216 0.793
Control 95.7 ± 4.1 281.4 ± 7.3 2.97 ± 0.11 1.21 ± 0.05
3-OH-ANA 96.0 ± 5.0 267.2 ± 4.2 2.84 ± 0.15 1.21 ± 0.05
XAN 91.7 ± 4.1 260.4 ± 3.5* 2.87 ± 0.13 1.23 ± 0.05
ANOVA, F 0.299 4.148 0.311 0.054
P 0.746 0.037 0.738 0.947

Notes: States are given in nmol O × min−1 × mg−1. Values represent means ± S.E.M; n = 6;

*

P < 0.05,

**

P < 0.01 in comparison to the respective control.