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The antifreeze activities of DAFP-1 (453μM) in the presence of NADH compared with those in the presence of trehalose (the previously identified most efficient naturally occurring enhancer) and TTHA (the previously identified most efficient enhancer). The TH values were presented as means, and the standard deviations were with 2% of the mean values. The concentrations of the enhancers were in the range of 0–0.80M. The pH of the samples was 7.5.
