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The antifreeze activities of DAFP-1 (453μM) in the presence of NADH, FAD, CoA, and the NAD(H) analogs (NAD, TNAD, and PAAD). The TH values were presented as means, and the standard deviations were with 2% of the mean values. The concentrations of the enhancers were in the range of 0–0.80M. The pH of the samples was 7.5. The inset shows the expansion of the low concentration region of the enhancers (0.06–0.10M).
