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A typical HPLC chromatogram of the binding of NADH to DAFP-1 using the Hummel and Dreyer method. Flow rate was 0.35ml/min. The buffer contained 0.10M Na2SO4, 0.05% NaN3, 0.10M NaPi, and NADH at 0.50μM, pH 7.00. 1.51μM DAFP-1 in the above buffer was injected into the equilibrated column. The positive peak was observed at the retention time of DAFP-1, 10.0min. The trough was observed at 11.5min, the retention time of NADH.
