Figure 13. PRDX2 expression is regulated by HIF-1 and HIF-2.

A. HeLa cells were exposed to 20% or 1% O₂ for 24 h. RT-qPCR assays were performed using primers specific for the indicated mRNAs. Data are shown as mean ± SEM, n = 3. ^***p < 0.001 versus 20% O₂. B. HeLa cells were exposed to 20% or 1% O₂ for the indicated time. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1α, HIF-2α, and actin. The PRDX2 and actin bands were quantified by densitometry and normalized to 0 h (20% O₂). Normalized data are shown as mean ± SEM, n = 3. ^*p < 0.05, ^***p < 0.001 versus 20% O2. C. HeLa-shSC (SC), HeLa-shHIF-1α(1α), HeLa-shHIF-2α(2α), and HeLa-sh1α+2α (DKD) cells were exposed to 20% or 1% O₂ for 72 h. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1α, HIF-2α, and actin.