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. 2016 Jan 27;7(6):6436–6447. doi: 10.18632/oncotarget.7042

Figure 3. THC treatment affects viability, proliferation and immunomodulatory effects of BM-MSCs.

Figure 3

a., b. THC treatment for 24 h at low concentration (0.5, 1 or 2 μM) had no effect on the viability and proliferation of BM-MSCs, while THC at higher concentrations (5 or 10 μM) significantly decreased cell viability and proliferation. Cell viability was measured by MTT assay. Proliferation was examined by CCK-8 assay. **p < 0.01 versus control (no THC treatment). c. Experimental diagram for MSC-CM collection and the following culture of primary microglia. Pre-treatment with 1 μM THC for 24 h significantly improved the immunomodulatory properties of BM-MSCs in primary microglia, as suggested by the lowest release of inflammatory cytokines in microglia when stimulated by LPS (100 ng/Ml, 24 h), including TNF-α d. IL-1β e. IL-6 f., IL-8 g. and IL-10 h. Data were presented as mean ± SEM. **p < 0.01 versus control group (the first column in d-h), #p < 0.05 and ##p < 0.01 versus LPS group (the second column in d-h).