Figure 6. A short in vitro treatment with rapamycin significantly improves the survival and antitumor activity of in vitro expanded tetramer⁺ CD8+ T cells.

A. Survival of adoptively transferred rapamycin-treated (Rap-T) and non-treated (Ctrl-T) tetramer⁺ CD8⁺ T cells at day 7 in mice with or without IL-15 treatment. B. Survival of IL-15-treated and non-treated mice that received injection i.v. of 1×10⁵ Mel 624 cells plus 5×10⁶ Ctrl-T (Mel/Ctrl-T and Mel/Ctrl-T/IL-15) or Rapa-T (Mel/Rapa-T and Mel/Rapa-T/IL-15) cells (n = 6 per group), and non-treated mice receiving 1×10⁵ Mel 624 cells only (Mel; n = 4). **, p < 0.01; ***, p < 0.001; n.s., not significant