Figure 5. Runx1 is required for tumor cell migration.

LM3 cells (A) and MDA-MB-231 cells (B) were subjected to wound healing assays for 18 h after transfection with DN or C expression vectors. Time points: 0 h and 18 h. A representative assay (up) and histograms (down) show mean and SD of three independent experiments (LM3 p = 0.02; MDA p = 0,0035). (C) LM3 and MDA-MB-231 cell number were assessed before and after transfection with DN or C expression vectors. Histogram shows mean and SD of three independent experiments (LM3 p = 0.0007; MDA p = 0,03) Axis X is 10⁵ cells. (D) After transfection with DN or C expression vectors, LM3 cells were incubated with Annexin V-FITC and PI. Apoptosis was measure by flow citometry. PFA 4% was used as positive control.