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. 2015 Oct 23;7(6):6576–6592. doi: 10.18632/oncotarget.5878

Figure 5. MLN0128 induces G0/G1 cell cycle arrest and cell death in MCC cells.

Figure 5

A. Increased cell cycle arrest in G0/G1 upon MLN0128 treatment. MCC cells were treated with MLN0128 for 24 hours at indicated concentrations, followed by BrdU and 7-AAD staining and flow cytometry analysis. B. Increased MCC cell death upon MLN0128 treatment. MCC cells were treated with MLN0128 for 24 hours at indicated concentrations, followed by Annexin V and propidium iodide (PI) staining and flow cytometry analysis cytometry analysis. C. Decreased expression of cyclin D1 and increased expression of p27 by Western blotting. MCC cells were treated with MLN0128 for 24 hours at the indicated concentrations. Tubulin was used as a loading control. D. Increased cleavage and activation of caspase-3, caspase-7 and PARP by Western blotting. MCC cells were treated with MLN0128 for 24 hours at indicated concentrations. Tubulin was used as a loading control. Data are presented as the mean ± SEM of triplicate experiments. *p < 0.05 compared with control cells.