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H460 cell pools stably expressing either non-targeting shGIPZ or shERK3 were transiently transfected with either non-targeting control siRNA (siCtrl) or siRNA specifically targeting TDP2 (siTDP2). 24 hrs post-siRNA transfection, cells were treated with either DMSO or etoposide for 2 days. PARP-1 cleavage was analyzed by Western blotting.
