Table 1. Prime and DNA substrates.
Name | Oligonucleotide sequence | Application |
---|---|---|
Pol β R152C F | 5′-gggactttgaaaaaagaattccttgtgaagagatgttacaaatg-3′ | Mutagenesis |
Pol β R152C R | 5′-ttgcatttgtaacatctcttcacaaggaattcttttttcaaagtccc-3′ | |
Pol β -2oli | 5′-ct tacacgttgact accgt 5′-ggatccactcttgcctcaaaagacggtagtcaacg tgtaag-3′ |
Polymerase activity |
Pol-β -3oli | 5′-ct tacacgttgact accgt ttttgaggcaagagt ggat cc-3 5′-ggatccactcttgcctcaaaagacggtagtcaacgtgtaa g-3′ |
Polymerase activity & DNA binding assay |
Pol β -U | 5′-c ttacacgttgac taccttUtttgaggcaagagtgga t cc-3′ 5′-ggatc cactc ttgcc tcaaa gaagg tagtcaacgt gtaag-3′ |
SP-BER assay |
Pol β -F | 5′-c ttacacgttgac tacctt Ftttgaggcaagagtgga tcc-3′ 5′-ggatccactcttgcctcaaagaaggtagtcaacgtgtaag-3′ |
LP-BER assay |