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A. A melanoma tissue array was stained using the TRAF6 antibody. IRIDI score was calculated and statistics analyzed as described in Materials and Methods. B. Representative images of immunohistochemical staining of TRAF6 in metastatic melanomas, primary melanomas and normal nevi were taken at 200× magnification. C. Immunoblot analysis was performed to examine TRAF6 expression in several normal and skin cancer cell lines by indicated antibodies.
