Figure 1. ART-838 inhibited human acute leukemia cell growth and colony formation, more potently than did AS.

A. 23 human leukemia cell lines were treated with increasing concentrations of ART-838 or AS for 48h, and then cytotoxicity was assessed via fluorescent alamarBlue assay. Following subtraction of background fluorescence from all wells, fluorescence of drug-treated cells was normalized to that of vehicle (<0.5% DMSO)-treated cells to determine the effect of each drug concentration on viability. Each cell line was tested in triplicate and in at least three independent experiments. IC₅₀ (concentration that inhibits cell growth by 50%) values were calculated using CompuSyn software (ComboSyn, Paramus, NJ). All IC₅₀s were determined using cells cultured in RPMI containing 10% FBS. In addition several cell lines were initially cultured and tested in alternative media (Supplementary Table S2). Bisecting lines indicate mean IC₅₀s and error bars, SEM (**, p<.01) B. MOLM14 (AML) or MV-4-11 (biphenotypic leukemia) cells were pretreated with the indicated concentrations of ART-838, AS, or vehicle (0.5% DMSO) for 24h then cultured in semi-solid medium, in triplicate, for 7-10d before colonies were enumerated. Colony counts were normalized to vehicle-treated controls (100%). Three independent experiments were performed.